A forensic scientist is performing genetic fingerprinting to analyze variable number tandem repeats (VNTRs) from a genomic DNA sample. Which of the following options shows the correct sequence of steps for this analysis?
Digest DNA with restriction endonucleases →\rightarrow→ Separate fragments by gel electrophoresis →\rightarrow→ Transfer fragments to a nylon membrane →\rightarrow→ Hybridise with a labelled DNA probe →\rightarrow→ Detect bands using autoradiography
Separate genomic DNA by gel electrophoresis →\rightarrow→ Digest DNA in the gel with restriction endonucleases →\rightarrow→ Transfer fragments to a nylon membrane →\rightarrow→ Hybridise with a labelled DNA probe →\rightarrow→ Detect bands using autoradiography
Digest DNA with restriction endonucleases →\rightarrow→ Hybridise with a labelled DNA probe in solution →\rightarrow→ Separate fragments by gel electrophoresis →\rightarrow→ Transfer fragments to a nylon membrane →\rightarrow→ Detect bands using autoradiography
Digest DNA with restriction endonucleases →\rightarrow→ Transfer genomic DNA to a nylon membrane →\rightarrow→ Separate fragments on the membrane using electrophoresis →\rightarrow→ Hybridise with a labelled DNA probe →\rightarrow→ Detect bands using autoradiography
39 exam-style questions on OCR A A Level Biology Cloning and biotechnology. Each one has a worked solution and a mark scheme showing where the marks go.