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Cloning and biotechnology

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Question 12

A researcher amplified a specific gene region using PCR and wanted to estimate the size of the resulting DNA fragments using agarose gel electrophoresis.

Which of the following options shows the correct procedure for this analysis?

Load the PCR product onto the agarose gel →\rightarrow→ apply an electrical voltage for a set duration →\rightarrow→ stain the gel with a fluorescent DNA-binding dye →\rightarrow→ visualize the bands under UV light

Load the PCR product onto the agarose gel →\rightarrow→ apply an electrical voltage for a set duration →\rightarrow→ visualize the bands under UV light →\rightarrow→ stain the gel with a fluorescent DNA-binding dye

Stain the agarose gel with a fluorescent DNA-binding dye →\rightarrow→ apply an electrical voltage for a set duration →\rightarrow→ load the PCR product onto the gel →\rightarrow→ visualize the bands under UV light

Visualize the agarose gel under UV light →\rightarrow→ load the PCR product onto the gel →\rightarrow→ apply an electrical voltage for a set duration →\rightarrow→ stain the gel with a fluorescent DNA-binding dye

Cloning and biotechnology Questions

  1. A Level
  2. /Biology
  3. /Cloning and biotechnology