To study the internal ultrastructure of cell organelles, such as ribosomes (diameter ≈22 nm\approx 22\text{ nm}≈22 nm) and the inner membranes of mitochondria, a high-resolution microscope must be selected.
Which row in the table below correctly identifies the microscope type required, the physical basis for its high resolution, and the resulting specimen conditions and image type?
| Microscope Type | Physical Basis for Resolution | Image Nature & Specimen State | |
|---|---|---|---|
| Row A | Transmission Electron Microscope | Wavelength of electron beam is much shorter than light | 2D internal ultrastructure; dead specimen |
| Row B | Scanning Electron Microscope | Wavelength of electron beam is much longer than light | 3D surface structure; living specimen |
| Row C | Transmission Electron Microscope | Electromagnetic lenses focus visible light to a smaller point | 3D internal structure; living specimen |
| Row D | Light Microscope | Light waves have a shorter wavelength than electron beams | 2D colour image; dead or living specimen |
Row A
Row B
Row C
Row D
