Collagen is a fibrous structural protein widely used in cosmetic formulations. To verify the quality of marine-derived collagen extracts, a biochemistry laboratory determines the protein concentration of their samples using a colorimetric assay.
To produce a calibration curve for this assay, you are provided with:

Use the information provided to describe how you could produce a calibration curve for collagen concentration. Do not include details on the use of specific glassware (such as pipettes or test tubes) in your answer.
Describe how you would determine the concentration of collagen in an unrefined marine extract sample using your calibration curve.