To model canine pancreatic lipase activity, a veterinary scientist incubated a simulated duodenal fluid containing a high-fat canine food emulsion with pancreatic lipase and bile salts in a reaction vessel at 37∘C37^\circ\text{C}37∘C. The scientist withdrew samples of the mixture at specific time intervals.
The concentrations of triglycerides and monoglycerides in the samples were measured, as shown in Table 1.
Table 1
| Sample | Incubation time / min\text{min}min | Concentration of monoglycerides / mmol dm−3\text{mmol dm}^{-3}mmol dm−3 | Concentration of triglycerides / mmol dm−3\text{mmol dm}^{-3}mmol dm−3 |
|---|---|---|---|
| X | 15 | 3.4 | 1.5 |
| Y | 45 | 5.2 | 0.0 |
Use your knowledge of lipid digestion to explain the differences in the results for samples X and Y shown in Table 1.
Immediately after withdrawing each sample, the scientist placed it in a water bath at 95∘C95^\circ\text{C}95∘C for 10 minutes. Explain why.
Describe the role of micelles in the absorption of monoglycerides and fatty acids into the epithelial cells of the small intestine.
