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Biological molecules

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Question 28

Whey protein isolate is a major component of sports nutrition shakes. A quality-control analyst needs to verify the protein concentration in a newly formulated recovery drink. They decide to use a colorimetric assay (using Biuret reagent) to construct a calibration curve.

To produce this calibration curve, the analyst is provided with:

  • A stock whey protein solution of known concentration (5.0 mg/cm35.0\text{ mg/cm}^35.0 mg/cm3)
  • Distilled water
  • Biuret reagent (which reacts with peptide bonds to form a purple-coloured complex)
  • A colorimeter with a suitable filter
a.

Describe how the analyst could use these materials to produce a calibration curve for whey protein concentration. Do not include details of specific glassware or pipetting techniques in your answer.

[4]
b.

Describe how the analyst would then determine the concentration of whey protein in a sample of the recovery drink using this calibration curve.

[2]

Biological molecules Questions

  1. A Level
  2. /Biology
  3. /Biological molecules