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Biological molecules

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Question 22

Protein (albumin) in the urine can be a sign of kidney disease. A colorimetric method can be used to determine the protein concentration in a urine sample by first creating a calibration curve of known protein concentrations.

To produce this calibration curve, you are provided with:

  • A protein solution of known concentration (10 mg/dm3)
  • Distilled water
  • A protein-detecting reagent (which reacts with protein to produce a purple-colored product)
  • A colorimeter
1.

Use the information provided to describe how you could produce a calibration curve for protein concentration. Do not include details on the use of glassware in your answer.

[4]
2.

Describe how you would determine the concentration of protein in a patient's urine sample using your calibration curve.

[2]

Biological molecules Questions

  1. A Level
  2. /Biology
  3. /Biological molecules